BIO21 one of Specialists Manufacturing CHITOSAN

How to produce a good quality of our fish scale OSSEIN product? 


Step 1 raw material preparation process - separate unknown material out from raw material (fish scale)

    • We have methods to separate the size of fish scale
      The purpose for  to separate the size of the fish scale (small, medium, big) 

    • Separate unknown materials out from fish scale by a separator machine and human.

Step 2 Raw material cleaning

    • Fish scales cleaning  by freshwater many times and for a long time to make sure that the fish scale clean

    • Fish scale cleaning with caustic soda (NaOH) to remove dust and fish mucus

      This process is critical to producing a good quality OSSEIN and by-product Tricalcium Phosphate. 

 

Step 3 Demineralization process

To extract calcium from fish scale and collect calcium for the Tricalcium phosphate product.

Step 4 Extraction other non-collagen proteins and pigmented out from fish scale

To purify the fish scale Ossein without non-collagen and pigmented and  adjustment rang of pH to be 6-8 

 

Step 5 Drying in the green house 

We have  a process drying in the greenhouse to protect contamination with unknown materials and quickly to dried.

Step 6 Fish scale Ossein QC and inspection

We have QC lab equipment to test ASH, Moisture, and pH. Inspection to remove unknown materials from the fish scale Ossein before using in-house Collagen production or shipping to the customer.

OSSEIN product properties specification and control

    • Moisture less than 15 %
      Moisture Content of the sample was determined by drying the sample at 105 c for 24 h or until the weight was constant. It was then calculated by the percentage of weight loss compared to the initial weight of the sample.
      Remark: this method was modified from AOAC 934.01.
      Drying Method ( A.O.A.C.1980 Page 125 )

    • pH 6-8
      PH Content of the sample was determined by storing the sample in hot water and stirring it for 10 minutes. It was then calculated by PH METER.

    • ASH less than 1 %
      Ash content of the sample was determined by storing the sample in a muffle furnace at 600 c for 24 h or until the weight was constant. Ash content was calculated from the percentage of ash residue compared to the initial weight of the moisture-free sample
      Remark: This method was modified from AOAC 938.08.
      Official Final Action Method ( A.O.A.C.1980 Page .125 )